Localization of 1,25-dihydroxyvitamin D3 in intestinal nuclei in vivo |
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Authors: | M Zile E C Bunge L Barsness S Yamada H K Schnoes H F DeLuca |
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Affiliation: | Department of Biochemistry, College of Agricultural and Life Sciences, and College of Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53706 USA |
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Abstract: | Autoradiography of frozen sections of intestinal tissue taken from rachitic chickens given a single intravenous dose of 1,25-dihydroxy[23,243H]vitamin D3 (650 pmol, 78 Ci/ mmol) has been carried out. Specific localization of label in the nuclei of intestinal villi and crypt cells could be demonstrated at 2.5 to 6 h postinjection. In contrast, no concentration or localization of radioactivity could be detected in intestinal muscle, liver, and skeletal muscle. These results strongly support the concept that the function of 1,25-dihydroxyvitamin D3 in intestine is mediated by a nuclear mechanism. |
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