Analysis of inactive X chromosome structure by in situ nick translation |
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Authors: | Karen A Dyer Donald Riley Stanley M Gartler |
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Institution: | (1) Departments of Medicine and Genetics and the Center for Inherited Diseases, University of Washington, 98195 Seattle, WA, USA |
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Abstract: | Nick translation assays of fixed interphase female fibroblasts with tritiated nucleotides demonstrated a characteristic absence of label over sex chromatin. The chromatin bodies were nearly always peripheral in location and a ribbon of nick translatable DNA was detected between the sex chromatin and the nuclear envelope. High voltage electron microscopy indicated the possibility of a special nuclear envelope attachment region. The apparent resistance of sex chromatin to nick translation did not appear to be due to resistance to DNase I attack. |
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