Protective effect of Bcl-2 in NSO myeloma cell culture is greater in more stressful environments |
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Authors: | B T Tey M Al-Rubeai |
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Institution: | (1) Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UM Serdang, Selangor, Malaysia;(2) Department of Chemical and Biochemical Engineering, University College Dublin, Belfield, Dublin 4, Ireland |
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Abstract: | In the present study, the protective effects of Bcl-2 over-expression in a suspension culture (without any adaptation) and
spent medium (low nutrient and high toxic metabolite conditions) were investigated. In the suspension culture without prior
adaptation, the viability of the control cell line fall to 0% by day 7, whereas the Bcl-2 cell line had a viability of 65%.
The difference in the viability and viable cell density between the Bcl-2 and control cell lines was more apparent in the
suspension culture than the static culture, and became even more apparent on day 6. Fluorescence microscopic counting revealed
that the major mechanism of cell death in the control cell line in both the static and suspension cultures was apoptosis.
For the Bcl-2 cell lines, necrosis was the major mode of cell death in the static culture, but apoptosis became equally important
in the suspension culture. When the NSO 6A1 cell line was cultured in spent medium taken from a 14 day batch culture, the
control cell line almost completely lost its viability by day 5, whereas, the Bcl-2 still had a viability of 73%. The viable
cell density and viability of the Bcl-2 cell line cultivated in fresh medium were 2.2 and 2.7 fold higher, respectively, than
those of the control cultures. However, the viable cell density and viability of the Bcl-2 cultivated in the spent medium
were 8.7 and 7.8 fold higher, respectively, than those of the control cultures. Most of the dead cells in the control cell
line were apoptotic; whereas, the major cell death mechanisms in the Bcl-2 cell line were necrotic. |
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Keywords: | apoptosis bcl-2 spent medium NSO myeloma cells necrosis |
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