| Abstract: | Literature values for ‘resting’ glycolytic and respiratory rates of guinea pig exudate polymorphonuclear leucocytes as reported by various authors were calculated to the same unit basis to determine what differences might exist. Comparison experiments investigated preparative techniques to determine the significance with respect to the variations discovered. Procedures using 12 or 0.1% casein in saline as peritoneal irritant, 0 or 37 °C collection temperatures, siliconed or non-siliconed glassware, and hemolysis treatment to remove red blood cells, did not affect glycolytic rate in leucocytes. Three different anticoagulants used, heparin, citrate, and ethylenediamine tetracetic acid (EDTA) did account for the variability in the reported glycolytic rates by increasing the lactate production as compared with cells not exposed to anticoagulant. Citrate and EDTA increased the lactate production approx. 65%, heparin 22%, with the increase apparently related to the calcium chelating ability. Calcium ions were found to depress the glycolytic lactate production proportionately as the concentration increased up to 0.0026 M in the incubation medium. The removal or absence of calcium ions from the medium increased the lactate formation. |
