Quantitative analysis of herbivore-induced cytosolic calcium by using a Cameleon (YC 3.6) calcium sensor in Arabidopsis thaliana |
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Authors: | Francesca Verrillo Andrea Occhipinti Chidananda Nagamangala Kanchiswamy Massimo E Maffei |
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Institution: | 1. Plant Physiology Unit, Dept. Life Sciences and Systems Biology, University of Turin, Italy;2. Research and Innovation Center Biology and Genomic of Fruit Plants, Fondazione Edmund Mach FEM San Michele all’Adige (TN), Italy |
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Abstract: | Ca2+ is a key player in plant cell responses to biotic and abiotic stress. Owing to the central role of cytosolic Ca2+ (Ca2+]cyt) during early signaling and the need for precise determination of Ca2+]cyt variations, we used a Cameleon YC 3.6 reporter protein expressed in Arabidopsis thaliana to quantify Ca2+]cyt variations upon leaf mechanical damage (MD), herbivory by 3rd and 5th instar larvae of Spodoptera littoralis and S. littoralis oral secretions (OS) applied to MD. YC 3.6 allowed a clear distinction between MD and herbivory and discriminated between the two larvae instars. To our knowledge this is the first report of quantitative Ca2+]cyt determination upon herbivory using a Cameleon calcium sensor. |
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Keywords: | Arabidopsis thaliana Spodoptera littoralis Cameleon YC 3 6 Calcium signaling Herbivory |
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