Proteomic analysis of Populus × euramericana (clone I-214) roots to identify key factors involved in zinc stress response |
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Authors: | Stefania Romeo Dalila Trupiano Andrea Ariani Giovanni Renzone Gabriella S. Scippa Andrea Scaloni Luca Sebastiani |
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Affiliation: | 1. BioLabs, Institute of Life Sciences, Scuola Superiore Sant’Anna, 56127 Pisa, Italy;2. Dipartimento di Bioscienze e Territorio, University of Molise, 86090 Pesche, IS, Italy;3. Proteomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147 Napoli, Italy |
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Abstract: | Contamination of soil and water by heavy metals has become a widespread problem; environmental pollution by high zinc (Zn) concentration occurs frequently. Although poplar (Populus spp.) has been identified as suitable for phytoremediation approaches, its response to high Zn concentrations are still not clearly understood. For this reason, we investigated the effects of Zn in Populus × euramericana clone I-214 roots by proteomic analysis. Comparative experiments were conducted on rooted woody cuttings grown in nutrient solutions containing 1 mM (treatment) or 1 μM (control) Zn concentrations. A gel-based proteomic approach coupled with morphological and chemical analysis was used to identify differentially represented proteins in treated roots and to investigate the effect of Zn treatment on the poplar root system. Data shows that Zn was accumulated preferentially in roots, that the antioxidant system, the carbohydrate/energy and amino acid metabolisms were the main pathways modulated by Zn excess, and that mitochondria and vacuoles were the cellular organelles predominately affected by Zn stress. A coordination between cell death and proliferation/growth seems to occur under this condition to counteract the Zn-induced damage. |
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Keywords: | AS, anthocyanidin synthase BCF, bioconcentration factors DHODH, dihydroorotate dehydrogenase DW, dry weight GS, glutamine synthetase IAA, indole-3-acetic acid IEF, isoelectrofocusing IPG, isoelectrofocusing pH gradient PR, pathogenesis-related RGR, relative growth rate ROS, reactive oxygen species VDAC, porin/voltage-dependent anion channel protein. |
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