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Rescue of heavy metal effects on cell physiology of the algal model system Micrasterias by divalent ions
Authors:Stefanie Volland  Elisabeth Bayer  Verena Baumgartner  Ancuela Andosch  Cornelius Lütz  Evelyn Sima  Ursula Lütz-Meindl
Institution:1. Plant Physiology Division, Cell Biology Department, University of Salzburg, Hellbrunnerstraße 34, 5020 Salzburg, Austria;2. Institute of Botany, Faculty of Biology, University of Innsbruck, Sternwartestraße 15, 6020 Innsbruck, Austria
Abstract:Recent studies have shown that metals such as copper, zinc, aluminum, cadmium, chromium, iron and lead cause severe dose-dependent disturbances in growth, morphogenesis, photosynthetic and respiratory activity as well as on ultrastructure and function of organelles in the algal model system Micrasterias denticulata ( Volland et al., 2011, Volland et al., 2012 and Andosch et al., 2012). In the present investigation we focus on amelioration of these adverse effects of cadmium, chromium and lead by supplying the cells with different antioxidants and essential micronutrients to obtain insight into metal uptake mechanisms and subcellular metal targets. This seems particularly interesting as Micrasterias is adapted to extremely low-concentrated, oligotrophic conditions in its natural bog environment.
Keywords:AA  ascorbic acid  Ca  calcium  Cd  cadmium  Cr  chromium  EELS  electron energy loss spectroscopy  ESI  electron spectroscopic imaging  Fe  iron  Fe-EDTA  Fe-ethylenediaminetetraacetic acid  Gd  gadolinium  GSH  glutathione  HPLC  high-performance liquid chromatography  Pb  lead  PS II  photosystem II  ROS  reactive oxygen species  TEM  transmission electron microscopy  SA  salicylic acid  UPLC-MS  ultra performance liquid chromatography&ndash  mass spectrometry  Zn  zinc
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