Determination of abscisic acid and its glucosyl ester in embryogenic callus cultures of Vitis vinifera in relation to the maturation of somatic embryos using a new liquid chromatography-ELISA analysis method |
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| Authors: | Marí a Jesú s Prado,Asier Largo,Cristina Domí nguez,Marí a Victoria Gonzá lez,Manuel Rey,Marí a Luz Centeno |
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| Affiliation: | 1. Departamento de Biología Vegetal y Ciencia del Suelo, Universidad de Vigo, Campus Universitario, 36310 Vigo, Spain;2. Departamento de Ingeniería y Ciencias Agrarias, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, Campus de Vegazana s/n, 24071 León, Spain;3. Departamento de Fisiología Vegetal, Universidad de Santiago de Compostela, Campus Sur, 15872 Santiago de Compostela, Spain |
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| Abstract: | |
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| Keywords: | 2,4-D, 2,4-dichlorophenoxyacetic acid ABA, abscisic acid AP, alkaline phosphatase ABH, p-aminobenzoyl hydrazone BA, benzyladenine HPLC, high-performance liquid chromatography IAA, indole-3-acetic acid MRM, multiple reaction monitoring mode NOA, naphthoxyacetic acid PBS, phosphate-buffered saline PGR, plant growth regulator PcAbs, polyclonal antibodies SPE, solid-phase extraction TBS, Tris-buffered saline UPLC, ultra-performance liquid chromatography |
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