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Construction of a gene of the human tumor-associated antigen VNTR(MUC1) bound to streptavidin, its expression inEscherichia coli, and the study of properties of the hybrid protein |
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| Authors: | L. B. Gul’ko O. V. Pavlova N. A. D’yakov N. A. Okorokova K. I. Ratmanova N. N. Logunova R. A. Bobreneva V. A. Makarov V. L. Yurin V. P. Veiko V. G. Debabov |
| Affiliation: | (1) State Research Center of Genetics and Selection of Industrial Microorganisms of the Russian Federation, Pervyi Dorozhnyi proezd 1, 113545 Moscow, Russia |
| Abstract: | A gene of human tumor-associated antigen VNTR(MUC1) bound to streptavidin, an expression plasmid, and a highly effective hybrid protein-producing strain were constructed. It was shown that the streptavidin leader peptide ensures effective secretion of the hybrid protein into the periplasmic space ofEscherichia coli cells. The hybrid protein was isolated in a homogeneous state and its immunogenic properties were studied. |
| Keywords: | Escherichia coli hybrid protein MUC1 streptavidin tumor-associated antigen VNTR |
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