| 野生种马铃薯SpPHYB基因的克隆与表达分析 |
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| 引用本文： | 张易,杨清,王全逸,郭建林,梁峰.野生种马铃薯SpPHYB基因的克隆与表达分析[J].植物生理学通讯,2007,43(6):1020-1024. |
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| 作者姓名： | 张易 杨清 王全逸 郭建林 梁峰 |
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| 作者单位： | 南京农业大学生命科学学院，南京农业大学生命科学学院，南京农业大学生命科学学院，南京农业大学生命科学学院，南京农业大学生命科学学院 南京 210095，南京 210095，南京 210095，南京 210095，南京 210095 |
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| 基金项目： | 国家转基因植物研究和产业化专项基金 |
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| 摘 要： | 采用RT-PCR技术从野生种马铃薯中克隆到一个光敏色素基因PHYB,其cDNA全长为3470bp。含有一个3393 bp的完整开放阅读框,编码一条长1130个氨基酸的蛋白,分子量为125kDa,等电点为5.6。该基因编码的蛋白序列与栽培种马铃薯、番茄和烟草同源基因编码的氨基酸序列一致性分别为98%、95%、92%,命名为SpPHYB.半定量PCR分析表明,根、茎、叶和芽中SpPHYB表达水平较高且相似,但在花和块茎成熟器官中表达量稍低.
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| 关 键 词： | 野生种马铃薯 克隆 表达 |
| 收稿时间： | 2007-07-09 |
| 修稿时间： | 2007-09-25 |
Cloning and Expression Analysis of SpPHYB Gene from Solanum pinnatisectum Dunal |
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| ZHANG Yi,YANG Qing,WANG Quan-Yi,GUO Jian-Lin,LIANG Feng.Cloning and Expression Analysis of SpPHYB Gene from Solanum pinnatisectum Dunal[J].Plant Physiology Communications,2007,43(6):1020-1024. |
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| Authors: | ZHANG Yi YANG Qing WANG Quan-Yi GUO Jian-Lin LIANG Feng |
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| Institution: | College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | The full-length cDNA of SpPHYB gene was cloned from wild potato (Solanum pinnatisectum) by RT-PCR.The cDNA was 3470 bp long with an open reading frame (ORF) of 3 393 bp and encoded 1 130 amino acid residues with a predicted molecular weight of 125 kDa and an isoelectric point of 5.6.The protein se- quence Comparison showed that the predicted protein had higher identities to those from Solanum tuberosum, Lycopersicon esculentum and Nicotiana tabacum (98%,95% and 92%).Semi-quantitive RT-PCR indicated that the expression level of SpPHYB was higher and similar in roots,stem,leaves and sprouts,and lower in flowers and tubers. |
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| Keywords: | SpPHYB |
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