Isolation of the murI gene from Brevibacterium lactofermentum ATCC 13869 encoding d-glutamate racemase |
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Authors: | Konganapuram C Malathia Masaaki Wachia Kazuo Nagaia |
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Institution: | Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan. |
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Abstract: | The murI gene encoding D-glutamate racemase plays an important role in the biosynthesis of D-glutamic acid, an essential component of cell wall peptidoglycan of almost all eubacteria. A DNA fragment that could rescue the auxotrophy of D-glutamic acid in the Escherichia coli murI mutant strain WM335 was isolated from Brevibacterium lactofermentum ATCC 13869 belonging to the coryneform bacteria. DNA sequencing reveals that it encodes a protein of 284 amino acid residues, which shows a high level of homology with D-glutamate racemases from several other bacteria. |
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Keywords: | Coryneform bacterium Brevibacterium lactofermentum Corynebacterium glutamicum d-Glutamate racemase murI |
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