Electrogenicity of the lysosomal proton pump |
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Authors: | P Dell'Antone |
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Institution: | CNR Unit for the Study of Physiology of Mitochondria, c/o Institute of General Pathology, University of Padova, Italy |
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Abstract: | Using acridine orange as a pH gradient probe, the effects of valinomycin and FCCP on the pH gradient across lysosomal membranes in an ATP-free medium as well as on the rate of the inward ATP-driven proton translocation were investigated. Both lysosome-enriched and highly purified lysosomal preparations from rat liver were used with identical results. Ionophore effects were found to be different depending upon whether passive ion fluxes or ATP-driven H+ translocation were involved and supported the existence of a membrane potential in the latter case. Anions stimulated the rate of ATP-driven proton translocation and stimulation increased with increasing anion lipophilicity. These results strongly support the electrogenic nature of the lysosomal proton pump. |
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Keywords: | Lysosome Proton transport ATPase Acridine orange Electrogenic proton pump (Rat liver) FCCP |
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