A reusable amperometric biosensor based on a novel silver-epoxy electrode for immunoglobulin detection |
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| Authors: | R.F. Dutra G.D. Coelho V.L. Silva W.M. Ledingham J.L. Lima Filho |
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| Affiliation: | (1) Departamento de Patologia, ICB – ESEF/UFPE, Recife – PE, Brazil;(2) Laboratório de Imunopatologia Keizo Asami, (LIKA)/UFPE, Recife – PE, Brazil;(3) Departamento de Engenharia Química/, UFPE, Recife – PE, Brazil;(4) Department of Life Science, University of St. Andrews, Scotland |
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| Abstract: | A renewable immunosensor consisting of an `epoxygraphite' biocomposite containing silver and tetracyanoquinodimethane (TCNQ) is described. These compounds enhance conductivity allowing the use of a smaller potential (0.28 v) which, in turn, enhances selectivity. This sensor, which may be renewed by simple polishing of its surface, was employed to detect human IgG using peroxidase-coupled anti-human IgG. |
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| Keywords: | biocomposite epoxy graphite immunoglobulin immunosensor TCNQ |
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