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A new primer for sequencing DNA fragments cloned in M13mp vectors |
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| Authors: | A N Boldyrev A N Siniakov V A Petrenko |
| Abstract: | Tridecadeoxyribonucleotide d(CCAGGGTTTTCCC) was prepared by solid phase crown-ether-catalyzed phosphotriester method and proposed a new sequencing primer. This primer expands capacities of the Sanger dideoxy-chain-terminating method to solve various sequencing problems as compared to well-known universal primers. Criteria of the choice of an oligonucleotide primer without computer analysis of nucleotide sequence are described. |
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