Structural analysis of mucoidan, an acidic extracellular polysaccharide produced by a pristane-assimilating marine bacterium, Rhodococcus erythropolis PR4 |
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Authors: | Urai Makoto Yoshizaki Hiroshi Anzai Hirosi Ogihara Jun Iwabuchi Noriyuki Harayama Shigeaki Sunairi Michio Nakajima Mutsuyasu |
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Affiliation: | Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-8510, Japan. |
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Abstract: | Rhodococcus erythropolis PR4 is a marine bacterium that can degrade various alkanes including pristane, a C(19) branched alkane. This strain produces a large quantity of extracellular polysaccharides (EPS), which are assumed to play an important role in the hydrocarbon tolerance of R. erythropolis PR4. The strain produced an acidic EPS, mucoidan, together with a fatty acid-containing EPS, PR4 FACEPS. The chemical structure of the mucoidan was determined using (1)H and (13)C NMR spectroscopy and by conducting 2D DQF-COSY, TOCSY, HMQC, HMBC, and NOESY experiments. The mucoidan was shown to consist of a pentasaccharide repeating unit with the following structure: [structure: see text]. |
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Keywords: | EPS, extracellular polysaccharide FACEPS, fatty acid-containing extracellular polysaccharides CTAB, cetyltrimethylammomium bromide TFA, trifluoroacetic acid ABEE, 4-aminobenzoic acid ethyl ester DQF-COSY, double quantum filtered-correlation spectroscopy TOCSY, total correlation spectroscopy HMQC, heteronuclear multiple-quantum coherence HMBC, heteronuclear multiple-bond coherence NOESY, nuclear Overhauser effect spectroscopy |
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