Crystallization and preliminary X-ray analysis of porin from Rhodobacter capsulatus |
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| Authors: | U Nestel T Wacker D Woitzik J Weckesser W Kreutz W Welte |
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| Affiliation: | Institut für Biophysik und Strahlenbiologie der Albert-Ludwigs-Universit?t, Freiburg i.Br., FRG. |
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| Abstract: | Porin monomers of the phototrophic bacterium Rhodobacter capsulatus were purified. Crystals were obtained from a solution of porin solubilized with the detergent octyltetraoxyethylene within 5 days using the vapor phase equilibration technique. The crystals were rhombohedral with an edge length of 0.4 mm. The space group was trigonal R3 with unit cell constants of a = b = 95 A, c = 147 A. Reflexions were observed to 3.2 A. |
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