软骨多糖诱导MCF-7乳腺癌细胞凋亡的实验研究

研究软骨多糖诱导MCF-7乳腺癌细胞凋亡及其作用机理。方法:选用MCF-7人类乳腺癌细胞系体外培养,应用MTT法检测细胞生长抑制率,TUNEL法检测细胞凋亡率,HE染色法观察细胞形态学改变,流式细胞仪检测细胞周期的变化,免疫荧光方法检测BCL-2BAD及波形蛋白Vimentin的表达率。结果:软骨多糖对MCF-7细胞体外生长具有明显的抑制作用,且呈时间和浓度依赖性;软骨多糖可诱导MCF-7细胞发生凋亡并伴随有凋亡小体出现等形态学变化;软骨多糖促进BCL-2蛋白的表达水平下降,BAD表达水平上升,及Vimentin的降解。结论:软骨多糖能够在体外诱导MCF-7细胞凋亡,是一种新型的抗乳腺癌活性物质。

Study on Anti-Breast Cancer Mechanism of Cartilage Polysaccharide

To study the apoptotic effect of cartilage polysaccharide on MCF-7 human breast cancer cells and its mechanism.Methods:MCF-7 human breast cancer cells were cultured in vitro,cell inhibition rate was measured by MTT assay,apoptotic rate was measured by TUNEL assay,changes in cell morphology were observed by HE staining,changes in cell cycle were measured by flow cytometry,expression of Bcl-2,Bad and Vimentin protein was measured by immunofluorescent assay.Results:Cartilage polysaccharide could greatly inhibit the growth of MCF-7 cells in vitro,with a time dependent and dose dependent manner.Cartilage polysaccharide could induce apoptosis of MCF-7 cells with morphological changes of apoptotic body presentation.The expression of Bcl-2 protein was down-regulated and the expression of Bad protein was up-regulated,as well as lysis of vimentin was stimulated induced by cartilage polysaccharide.Conclusions:Cartilage polysaccharide induces apoptosis in MCF-7cells in vitro and it is a novel anti-breast cancer agent.