哈茨木霉与绿色木霉对杉木种子萌发和幼苗生长的影响

以杉木种子为材料,研究不同浓度(0.003、0.03、0.3、3、30、300 mg·L^-1)哈茨木霉和绿色木霉溶液对杉木种子萌发和幼苗生长的影响.结果表明: 各浓度木霉溶液处理对杉木种子萌发和幼苗生长均有一定的促进作用,其促进效果随着处理浓度的增加均呈先升后降的趋势.与对照相比,0.03 mg·L^-1哈茨木霉和绿色木霉处理对提高杉木种子的发芽率、发芽势、发芽指数、活力指数、根长、苗高和鲜质量效果最佳,分别提高了57.6%、125.0%、51.0%、209.2%、114.3%、16.1%、24.6%和42.7%、76.7%、43.9%、185.4%、113.8%、8.6%、22.6%;0.3 mg·L^-1哈茨木霉和绿色木霉显著提高杉木幼苗超氧化物歧化酶和过氧化物酶活性,分别增加了157.6%、179.9%和127.5%、116.2%,而丙二醛含量降低了86.1%和72.4%. 0.03～0.3 mg·L^-1浓度的哈茨木霉和绿色木霉不仅能显著促进杉木种子的萌发和幼苗生长,而且能够提高其抗氧化酶活性,增强杉木幼苗的抗逆性.

Effects of Trichoderma hazianum and T. viride on seed germination and seedling growth of Chinese fir.

Various concentrations of Trichoderma hazianum and T. viride treatments (0.003, 0.03, 0.3, 3, 30, 300 mg·L^-1) were designed to investigate their effects on seed germination and seedling growth of Chinese fir. The results showed that seed germination and seedling growth were signifi-cantly affected by all concentrations of both Trichoderma, and they presented to increase and then fall with the increasing treatment concentrations. The seed germination rate, germination energy, germination index, vigor index, seedling root length, shoot height and fresh mass reached their maxima at the 0.03 mg·L^-1 treatment. Compared with the control, the increments were 57.6%, 125.0%, 51.0%, 209.2%, 114.3%, 16.1% and 24.6% for T. hazianum, and 42.7%, 76.7%, 43.9%, 185.4%, 113.8%, 8.6% and 22.6% for T. viride, respectively. SOD and POD activities of Chinese fir seedlings reached their maxima, while MDA content reached the minimum at 0.3 mg·L^-1 treatment. Compared with the control, SOD and POD increased by 157.6% and 179.9%, while MAD decreased by 86.1% for T. hazianum, and SOD and POD increased by 127.5% and 116.2%, while MAD decreased by 72.4% for T. viride. This study indicated that 0.03-0.3 mg·L^-1 T. harzianum and T. viride could not only promote seed germination and seedling growth, but also improve the activities of antioxidant enzymes and the resistance of Chinese fir seedlings.