土耳其斯坦叶螨多重抗性品系选育及解毒酶活性的变化

为探索土耳其斯坦叶螨的多重抗药性及其生化机理,在室内对敏感系(SS)土耳其斯坦叶螨分别用螺螨酯、甲氰菊酯和阿维菌素的混剂进行处理,选育出多重抗性品系(Mp-R).结果表明: 选育至15代,土耳其斯坦叶螨的抗性指数达35.74倍.对不同品系的解毒酶活性分析显示,Mp-R品系相对SS品系的羧酸酯酶(CarE)、谷胱甘肽-S-转移酶(GSTs)和多功能氧化酶(MFO)的比活力分别是SS品系的1.21、1.53、9.18倍.说明CarE、GSTs、MFO的活性升高可促进土耳其斯坦叶螨对3种杀虫剂多重抗性的形成;MFO的活性升高可能是土耳其斯坦叶螨对3种杀虫剂产生多重抗性的主要原因.测定Mp-R品系和单抗品系(Ip-R)的农药感性和解毒酶活力变化发现,3种杀虫剂的混合使用可能会延缓土耳其斯坦叶螨对甲氰菊酯的抗性形成,加快对阿维菌素的抗性形成.

Multiple resistance selection with insecticides and change of detoxification enzyme activities in Tetranychus turkestani Ugarov et Nikolski (Acari: Tetranychidae).

In order to explore the multi-pesticide resistance and biochemical mechanism of Tetranychus turkestani, the related susceptible strains (SS) of T. turkestani were treated by mixture of spirodiclofen, fenpropathrin and abamectin to breed multiple resistance strains (Mp-R) in the laboratory. The results showed that the multiple resistance of T. turkestani had reached 35.74 folds after 15 generations. The analysis on the detoxification enzyme activity of different strains showed, compared with the susceptible strains, the carboxyl esterase (CarE), glutathione-s-transferase (GSTs) and the mixed function oxidase (MFO) of the multiple resistance strains had reached 1.21, 1.53 and 9.18 folds, respectively, suggesting the increased activities of CarE, GSTs and MFO could induce the formation of multiple resistance to three insecticides among which MFO might be the main one. By comparing the pesticide sensitivity and detoxification enzyme activity of Mp-R and Ip-R, it was showed that continuous mixing use of the three pesticides might slow the resistance of T. turkestani to fenpropathrin, but accelerate its resistance to abamectin.