Callus induction and plant regeneration in Vetiveria zizanioides |
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Authors: | Marco Mucciarelli Marisa Gallino Silvano Scannerini Massimo Maffei |
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Affiliation: | (1) Department of Plant Biology, University of Torino, Viale P.A. Mattioli, 25, I-10125 Turin, Italy;(2) Department of Morphophysiology, University of Torino, Viale P.A. Mattioli, 25, I-10125 Turin, Italy |
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Abstract: | Callus induction was obtained from basal parts of Vetiveria zizanioides Stapf. leaves cultured on Murashige and Skoog (MS) medium supplemented with 9.0 M 2,4-dichlorophenoxyacetic acid (2,4-d), 5.7 M indoleacetic acid (IAA) and 4.6 M kinetin. Calli were maintained on MS medium with the addition of 0.9 M 2,4-d and 2.3 M kinetin. Shoot formation was obtained from fast growing 14-day-old callus on the same basal medium supplemented with 0.9 M 2,4-d and 9.3 M kinetin. Embryo-like structures were observed. When transferred to basal medium, shoots readily developed roots. Fully developed regenerated plants were then successfully established in soil. |
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Keywords: | callus maintenance embryogenesis Vetiver Poaceae |
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