Pseudosecretion of Escherichia coli chloramphenicol acetyltransferase by Bacillus subtilis. |
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Authors: | S F Le Grice R Gentz W Bannwarth H P Kocher |
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Abstract: | Bacillus subtilis harboring the vector 25RBSII secrets an Escherichia coli-derived chloramphenicol acetyltransferase into culture supernatants. The secreted enzyme lacks 18 amino acids; these are removed externally rather than during secretion. |
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