Vanadate reduction by molybdenum-free dissimilatory nitrate reductases from vanadate-reducing bacteria |
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Authors: | Antipov A N Lyalikova N N Khijniak T V L'vov N P |
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Institution: | A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow. |
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Abstract: | Molybdenum- and molybdenum cofactor-free nitrate reductases recently isolated by us from vanadate-reducing bacteria Pseudomonas isachenkovii are likely to mediate vanadate reduction. During anaerobic growth of P. isachenkovii on medium supplemented with nitrate and vanadate, vanadate dissimilation was followed by nitrate consumption, and this process was associated with some structural reorganizations of nitrate reductases. The homogeneous membrane-bound nitrate reductase of P. isachenkovii reduced vanadate with NADH as an electron donor. |
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