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The Fate of Mitochondria in Ibex-hirus Reconstructed Early Embryos |
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| Authors: | Yan JIANG Shu-Zhen LIU Yan-Ling ZHANG Man-Xi JIANG Qing-Yuan SUN and Da-Yuan CHEN State Key Laboratory of Reproductive Biology |
| Affiliation: | Yan JIANG,Shu-Zhen LIU,Yan-Ling ZHANG,Man-Xi JIANG,Qing-Yuan SUN,and Da-Yuan CHEN* State Key Laboratory of Reproductive Biology,Institute of Zoology,the Chinese Academy of Sciences,Beijing 100080,China |
| Abstract: | Inter-species nuclear transfer could be used to preserve North Goat (Capra ibex), an endan- gered species. We established the culture conditions for ibex-hirus reconstructed embryos and optimized the method for DNA extractions of a single cell and early cloned embryo. By using mitochondria-specific probes of ibex and hirus respectively we found that mitochondria of donor cells can co-exist with recipients in 1-cell and 2-cell stages of the reconstructed embryos but not in the following developmental stages. |
| Keywords: | Capra ibex cloned embryos nuclear transfer mitochondria PCR |
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