Sensitive and specific detection of Xanthomonas campestris pv campestris by PCR using species-specific primers based on hrpF gene sequences |
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Authors: | Park Young Jin Lee Byoung Moo Ho-Hahn Jang Lee Gil Bok Park Dong Suk |
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Institution: | National Institute of Agricultural Biotechnology, Rural Development Administration, 441-707 Suwon, Republic of Korea |
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Abstract: | A sensitive and specific assay was developed to detect bacterial black rot of crucifers caused by Xanthomonas campestris pv. campestris (X. c. pv. campestris), in cabbage seed and plant. Primers XCF and XCR from hrpF homologous to nolX, host recognition protein, were used to amplify a 525 bp DNA fragment. PCR technique was applied to detect the pathogen in naturally infected seed and plant of cabbage. The PCR product was only produced from X. c. pv. campestris among 40 isolates of Xanthomonas strains, Escherichia coli (O157:H7), Pectobacterium carotovorum subsp. carotovorum, and other reference bacteria. |
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Keywords: | hrpF PCR Specific primer Xanthomonas campestris pv campestris |
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