Repeated sequences with unusual properties from the divergent region of Crithidia oncopelti maxicircle kinetoplast DNA |
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Authors: | Ivan A. Tarassoff Evgeny V. Kuzmin Igor V. Levchenko Galina N. Zaitseva |
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Affiliation: | (1) Biology Dept., Moscow State University, Chair of Molecular Biology, 117234 Moscow, USSR |
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Abstract: | Summary The occurrence of bacterial promoter-like sequences was shown in the divergent region ofCrithidia oncopelti maxicircular kinetoplast DNA. A promoter-cloning vector based on the neomycin phosphotransferase II (NPT II) gene was used to localize promoters in three homologous blocks of repeated sequences. The elements found displayed greater promoter strength inEscherichia coli than the normal promoter of the NPT II gene. Sequences of three promoter-containing inserts from different blocks were determined and typical prokaryotic promoter sequences were localized. |
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Keywords: | Kinetoplast DNA Repeats Promoter-cloning vector Neomycin phosphotransferase II Prokaryotic promoters |
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