金佛拟小鲵幼体皮肤的显微结构观察

采用光镜和扫描电镜对金佛拟小鲵(Pseudohynobius jinfo)幼体皮肤进行组织学和形态学观察。金佛拟小鲵幼体皮肤由表皮和真皮构成。不同部位皮肤厚度不同,头部背侧皮肤最薄,其厚度为(45.99±12.77)μm,尾部腹侧的皮肤最厚,其厚度为(95.21±42.72)μm。表皮角质层仅躯干背部和尾部明显,由仍具有一定生理活性的复层扁平上皮细胞构成。皮肤腺体包括黏液腺和颗粒腺。黏液腺广泛分布于身体各个部位的皮肤,颗粒腺呈区域性分布,仅见躯干部和尾部皮肤,其体积大于黏液腺。毛细血管多分布于真皮疏松层腺体周围,与表皮层紧密接触并凸向表皮。色素细胞主要分布于表皮和疏松层的交界处,呈多细胞聚集的状态,形成厚度不一的色素层。

Skin Microstructure of Larval Pseudohynobius jinfo

The morphological and histological observation on the skin of larval Pseudohynobius jinfo was conducted by optical and scanning electron microscopy.Measurements were performed using image software Image-Pro Plus 6.0.All statistical tests were performed using software SPSS 19.0.All values given were shown as Mean ± SE.P ＜ 0.05 was considered significant in t test.The skin ofP.jinfo consisted of epidermis and dermis (Fig.1,3),but the thicknesses were different at different positions of the body (Table 1).The skin of the back head was the thinnest (45.99 ± 12.77 μm) and the ventral side of tail was the thickest (95.21 ± 42.72 μm).The stratum corneum consisted of stratified squamous epithelium cells with biological activity only found in the skin of tail and the back of trunk (Fig.1).The skin of the jaw and that of ventral side of trunk were different from other parts (Fig.2).Both mucus glands and granular glands were found (Table 2).Mucus glands widely spread over the body.Granular glands were distributed in the skin of trunk and tail.The capillary vessels were abundant around glands in stratum spongiosum and moved outside to make the epidermis thinner.Pigment cells were mainly distributed in stratum spongiosum near the epidermis (Fig.3).