普通菜豆生长习性相关基因定位

普通菜豆生长习性是一个重要的驯化性状。为定位生长习性相关基因,本研究选用无限蔓生型育成品种连农紫芸一号和有限丛生地方品种兔子腿配置杂交组合,构建F2分离群体和F2∶3家系。遗传分析表明,有限直立对无限蔓生是由1对隐性单基因控制,将该基因命名为gh-lz。利用分离群体分组分析法初步将该基因定位在B01连锁群,通过扩大群体和新开发的SSR、In/Del标记进一步将目的基因定位在SSR标记p1t52和In/Del标记In93之间,位于第1条染色体上45453003~45575103 bp之间,区间大小为122100 bp,预测候选区段共包含12个基因,命名为Gene1~Gene12。其中,Gene12为普通菜豆基因TFL1。本研究为普通菜豆生长习性相关基因的定位及进一步的功能研究奠定了分子基础。

Mapping of Growth Habit Related Gene in Common Bean

Growth habit is an important trait associated with the domestication in common bean(Phaseolus vulgaris L.),which is a component trait including the types of inflorescence and stem.To locate the growth habit gene, we established F2 plants and F2:3 plants families derived from a cross between indeterminate viny common bean cultivar LiannongziyunNO.1(LianziNo.1) and determinate bush landrace Tuzitui (F0404).The genetic analysis showed that determinate bush was controlled by a single recessive gene, designed as gh-lz, which was primarily mapped on B1 linkage group by bulked segregant analysis. With a lager mapping population, more SSR and In/Del markers, the gh-lz gene was further mapped between SSR marker p1t52 and In/Del marker In93, sited on 45,453,003bp-45,575,103bpon B01, with a physical region about 122kb. This region included 12 genes, named Gene1~Gene12.The 12 candidategenes were annotated by BLAST searching NCBI, and Gene12 was annotated as TFL1 in common bean.These results provided a foundation for growth habit trait study and gene cloning.