Divergicin 750, a novel bacteriocin produced by Carnobacterium divergens 750 |
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Authors: | Askild Hoick Lars Axelsson Ulrich Schillinger |
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Affiliation: | MATFORSK, Norwegian Food Research Institute, Osloveien 1, N-1430 Ås, Norway; Bundesforschungsanstalt für Ernährung, Karlsruhe, Germany |
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Abstract: | Abstract Divergicin 750, a bacteriocin produced by Carnobacterium divergens 750, preferentially inhibited the growth of strains of Carnobacterium and Enterococcus . Selected strains of Listeria monocytogenes and Clostridium perfringens were also inhibited. The bacteriocin was purified to homogeneity by ammonium sulfate precipitation and sequential S-Sepharose, hydrophobic interaction and reversed phase chromatography. The complete amino acid sequence was determined by Edman degradation. The peptide consisted of 34 amino acid residues. The calculated ifMr from the peptide sequence, 3447.7, agreed well with that obtained by mass spectrometry. Divergicin 750 did not show any sequence similarities to other known bacteriocins. The plasmid-located structural gene encoding divergicin 750 ( dvn750 ) was cloned and sequenced. The gene encoded a primary translation product of 63 amino acids with a deduced M rmr = 6789.4 which is cleaved between amino acid residues 29 and 30 to yield the mature bacteriocin. |
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Keywords: | Lactic acid bacteria Carnobacterium divergens Bacteriocin Protein purification Antimicrobial peptide Cloning |
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