Mutagenesis studies on the iron binding ligands of clavaminic acid synthase |
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Authors: | Doan L X Hassan A Lipscomb S J Dhanda A Zhang Z Schofield C J |
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Institution: | The Oxford Centre for Molecular Sciences, Oxford University, South Parks Road, Oxford, OX1 3QY, United Kingdom. |
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Abstract: | Mutagenesis studies on conserved histidine residues identified as possible metal binding ligands in clavaminic acid synthase isozyme 2 were consistent with His-145 and His-280 acting as iron ligands, in support of crystallographic and previous mutagenesis studies. Mutagenesis of the four cysteines and a glutamine residue, conserved in both clavaminic acid synthase isozymes 1 and 2, demonstrated that none of these residues is essential for activity. |
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