Choline derived from the phosphatidylcholine specific phospholipase D is not directly available for the CDP choline pathway in phorbol ester- treated C3H10T1/2 C18 fibroblasts |
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Authors: | Thorsen Vidar AT Bruland Ove Lillehaug Johan R Holmsen Holm |
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Institution: | (1) Department of Biochemistry and Molecular Biology, University of Bergen, Bergen, Norway |
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Abstract: | We have shown that 12-O-tetradecanoylphorbol 13-acetate (TPA) increases protein kinase C (PKC)-mediated choline transport, incorporation of choline into phosphatidylcholine (PtdCho) and PtdCho degradation by phospholipase D (PLD) in C3H10T1/2 Cl 8 cells. Dual prelabeling experiment using 3H]/14C]choline indicated that intracellular choline generated from the PLD reaction was not directly recycled to PtdCho synthesis within the cell, and that a large fraction of the choline was transported out of the TPA-treated cells. In contrast, medium derived choline was preferably channeled to PtdCho synthesis. These results indicate that in TPA-treated cells, the choline derived from the PKC-mediated increased PLD activity and the choline newly taken up by the cell behave as two distinctly different metabolic pools. |
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Keywords: | C3H10T1/2 Cl 8 cell line phosphatidylcholine cycle phospholipase D 12-O-tetradecanoylphorbol 13-acetate choline protein kinase C |
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