Growth ofZymomonas on lactose: Gene cloning in combination with mutagenesis |
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| Authors: | Steven E Buchholz Margaret M Dooley Douglas E Eveleigh |
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| Institution: | (1) Department of Biochemistry and Microbiology, Cook College, Rutgers University, New Brunswick, NJ, U.S.A.;(2) Present address: Experimental Station, E228/339, E.I. du Pont de Nemours & Co., 19898 Wilmington, DE, U.S.A.;(3) Present address: Biology Department, The College of Staten Island, 10301 Staten Island, NY, U.S.A. |
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| Abstract: | Summary Wild-type strains ofZymomonas mobilis have a limited substrate range of glucose, fructose and sucrose. In order to expand this substrate range, transconjugants ofZ. mobilis containing Lac+ plasmids have been constructed. Although  -galactosidase is expressed in such strains, they lack the ability to grow on lactose. We now report the development ofZ. mobilis strains capable of growth on lactose. This was achieved in two stages. First, a broad host range plasmid was constructed (pRUT102) which contained the lactose operon under the control of aZ. mobilis promoter plus genes for galactose utilization.Z. mobilis CP4.45 containing pRUT102 was then subjected to mutagenesis combined with continued selection pressure for growth on lactose. One strain,Z. mobilis SB6, produced a turbid culture that yielded 0.25% ethanol from 5% lactose (plus 2% yeast extract) in 15 days. |
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| Keywords: | Zymomonas mobilis Growth on lactose Cloning Mutation |
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