The importance of surface charge and hydrophobicity for the flocculation of chain-forming brewing yeast strains and resistance of these parameters to acid washing |
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| Authors: | Kate L Wilcocks Katherine A Smart |
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| Institution: | Departamento de Genétics, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, P.O. Box 83, 13418-900, Piracicaba, SP, Brazil; Cenargen/Embrapa, 70849-970, Brasìlia, DF, Brazil; Departamento de Biologia Celular, Universidade de Brasília, 70910-900, Brasília, DF, Brazil |
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| Abstract: | Abstract This paper describes transformation of intact conidia of Aspergillus nidulans , auxotrophic for arginine, by using the biolistic process. The plasmid employed was pFB39, carrying the argB gene. The transformation frequency obtained was 81 transformants/ μg of DNA. Classical genetics and molecular analysis were conducted to analyse transformants and to determine in which chromosome integration took place. |
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| Keywords: | Aspergillus nidulans Fungal transformation Biolistic gene transfer Conidial transformation |
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