蛋白芯片法在抗核抗体检测中的临床应用

目的：应用蛋白芯片法与欧蒙斑点法检测血清抗核抗体，并对其结果进行比较分析。方法：收集95例血清样本，其中45例确诊为自身免疫性疾病，阴性50例，同时采用蛋白芯片法和欧盟斑点法检测其抗核抗体（SSA、SSB、Sm、RNP、Scl—70、Jo-1、CENPB），并对结果进行对比分析。结果：45例确诊病例中，蛋白芯片法阳性44份，欧蒙斑点法阳性41份，敏感度分别为97．78％和91．11％，特异度分别为98．95％和95．79％；按卡方检验进行结果统计，x^2=1．75，P〉0．05，差异无统计学意义。结论：蛋白芯片可用于临床检测自身免疫性疾病、治疗监测、疗效评估和预后判断。

Clinica Application of Protein Array Analysis in Antinuclear Anti-body Tests

Objective： To compare antinuclear antibody tests for autoimmune disease with protein array analysis and EUROASSAY. Methods： Serum specimens from 45 patients with autoimmune disease and 50 negtive samples were tested for antinuclear antibody（SSA, SSB, Sm, RNP, Scl-70, Jo-1, CENPB） by protein array analysis and EUROASSAY. Results： In 95 serum specimens, 44 samples tested by protein array analysis were positive with the rate of 97.78%, while 41 samples tested by EUROASSAY were positive with the rate of 91.11%, no significant dif- ference was found between the two method（X^2=l.75, P〉0.05）. Conclusion： Protein chip may use in clinical auto-immune disease test, treatment monitoring, curative effects evaluation and prognosis estimation.