Activity and Isoenzyme Pattern of Lactate Dehydrogenase in Neurons and Astroblasts Cultured from Brains of Chick Embryos |
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Authors: | G. Tholey B. F. Roth-Schechter P. Mandel |
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Affiliation: | Laboratoire de Biochimie Microbienne, Facultéde Pharmacie, UniversitéLouis Pasteur, Strasbourg, France;Laboratoire de. Pharmacodynamie, Facultéde Pharmacie, UniversitéLouis Pasteur, Strasbourg, France;Centre de Neurochime du CNRS, 11, rue Humann, 67085 Strasbourg Cedex, France |
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Abstract: | Primary cultures of neurons and glial cells (astroblasts) prepared from brains of 8-day-old and 15-day-old chick embryos, respectively, were grown for periods between 3 and 19 days. Specific activity of lactate dehydrogenase (LDH) increased in both types of cultures as a function of time and was always significantly higher in glial cells than in neurons. Glial cell extracts were found to contain predominantly the anaerobic isoenzymatic form of LDH (LDH-H4), and this pattern did not change over a period of 19 days. Cultured neurons contained predominantly the aerobic isoenzymatic form LDH-H4, and there was a progressive appearance of all other isoenzymes over an 8-day period. These results support the hypothesis of a different energy metabolism in neurons and glia. |
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