Diel variability in nitrogenous nutrient uptake at photic and subphotic depths

Diel patterns in nitrogen uptake were investigated at threepositions along an offshore orientated transect in the SouthernBenguela Upwelling System. Both photic and sub-photic depthswere targeted in the ¹⁵N tracer experiments. An appreciableproportion of nitrogen uptake was found to occur at sub-photicdepths during both the day and the night. The importance ofsub-photic uptake decreased offshore but remained non-trivialat all locations. Although NH₄ dominated this uptake, NO₃ removalat depth did account for an important fraction of the totalwater column NO₃ uptake, particularly over the shelf at night.For example, sub-photic NO₃ uptake at night for the inshorestations averaged 68% of the total for the water column decliningto 3% offshore. The impact of sub-photic NO₄ uptake on new productionwill depend on whether the NO₃ is incorporated into primaryassimilation pathways or is transported to the sediments ininorganic form. Whereas sub-photic/photic uptake decreased offshore,night/day uptake ratios increased with distance from the coast,approaching unity at the offshore location. This effect wasmost obvious for the photic communities This finding supportsthe belief that dark nitrogen uptake assumes increased importanceas nutrients become less available. Photic zone f-ratios weresmaller at night (mean = 0.14) than during the day (mean = 0.28)indicating a greater importance of reduced nitrogen uptake duringthe dark hours This emphasis on NH₄ utilization at night canbe explained purely in terms of energy efficiency. Such die1phasing in f-ratios needs to be accounted for in scaling totalproduction to new proifudon.