植物激素在小球藻异养培养中的作用

本文研究了几种植物激素对小球藻异养培养的影响。结果表明 ,IAA、IBA及 6_BA三种植物激素均不同程度地促进了小球藻的异养生长 ,培养≤ 36h时 ,IAA或IBA以 2 0mg/L的促进小球藻异养生长的效应最大 ,1 0 0mg/LIAA或IBA则抑制了藻的生长 ;>36h时 ,1 0 0mg/LIAA或IBA表现出促进小球藻生长的效应 ,并最终获最大净A540 增长量 ;6_BA以 0 1mg/L的促进作用最大。IBA与 6_BA组合同样表现出促进小球藻异养生长的效应 ,但并非IBA和 6_BA简单的加合效应 ,5mg/LIBA与 6_BA组合的效应维持 6_BA单因子的作用趋势 ,2 0mg/LIBA与 1mg/L 6_BA组合的效应大于与 0 .1mg/L 6_BA组合的 ,1 0 0mg/LIBA与 0 .1mg/L 6_BA组合的效应在≤ 36h时大于与 1mg/L 6_BA组合的 ,>36h时则相反。另外 ,高浓度IBA(≥ 2 0mg/L)与 6_BA组合抑制了前中期异养藻对葡萄糖的吸收 ,但加速了中后期葡萄的吸收。再者 ,IBA与 6_BA组合加速了异养小球藻对NO_3 的吸收。

Roles of Phytohormones in the Growth of Chlorella vulgaris in Heterotrophic Culture

The effects of several kinds of plant hormones on heterotrophic culture of Chlorella vulgaris were researched. The results showed that plant hormones such as IAA, IBA and 6_BA could all stimulate the heterotrophic growth of Chlorella vulgaris in the heterotrophic culture to degrees. Within the culture time of 36 h, 20 mg/L IAA or IBA had the maximum stimulating effect on the heterotrophic growth. 100 mg/L IAA or IBA inhibited the heterotrophic growth within the culture time of 36 h but stimulated it after 36 h, and in the end the largest A 540 net increase was obtained by the treatment of 100 mg/L IAA or IBA. 6_BA enhanced the heterotrophic growth with the optimum concentration being 0.1 mg/L. The combination of IBA with 6_BA also stimulated the heterotrophic growth, but the stimulating effect was different from the simple adding effect of both IBA and 6_BA. The effect of the combination of 5 mg/L IBA with 6_BA was identical to the solely 6_BA effect. The combination of 20 mg/L IBA with 1 mg/L 6_BA had a greater stimulating effect than that of the combination of 20 mg/L IBA with 0.1 mg/L 6_BA. Moreover, within the culture time of 36 h, the effect of the combination of 100 mg/L IBA with 0.1 mg/L 6_BA was greater than that of the combination of 100 mg/L IBA with 1 mg/L 6_BA. However, after 36 h, the opposite was true. Furthermore, the results indicated that the combination of IBA equal to or more than 20 mg/L with 6_BA inhibited the uptake of glucose by the algae during the early middle culture stages and enhanced the uptake during the middle _late culture stages and that the combination of IBA with 6_BA stimulated the uptake of NO - 3 by the algae.