Initiation and Growth of Callus and Cell Suspensions of Theobroma cacao L |
| |
Authors: | TSAI C H; KINSELLA J E |
| |
Institution: | Institute of Food Science, Cornell University Ithaca, New York 14853, U.S.A. |
| |
Abstract: | Callus and suspension cultures of Theobroma cacao L., initiatedfrom immature cotyledons of beans from pods harvested 120130days after pollination were established. A modified B-5 or MurashigeSkoogagar medium sustained growth of callus without loss of vigourafter each sub-culture. A 15-fold weight increase occurred duringthe 4 week culture periods at 30 ± 1 °C. Coconutwater improved callus growth substantially. The optimum hormonalconcentrations for growth of suspensions were 0.5 mg 11of 2, 4-dichlorophenoxyacetic acid and 0.1 mg I1 of kinetinin a MurashigeSkoog basal medium liquid medium. The optimumtemperature for growth of suspensions was 2530 °C.The cell number and cell mass of suspensions increased 20-foldin 14 days. No organogenesis or embryogenesis was observed. Theobroma cacao L., acao, cell culture, suspension culture, tissue culture. |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|