Substrate selectivity of lipases in the esterification of cis/trans-isomers and positional isomers of conjugated linoleic acid (CLA) |
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Authors: | Siegfried Warwel Robert Borgdorf |
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Institution: | (1) Federal Centre for Cereal, Potato and Lipid Research, Institute for Biochemistry and Technology of Lipids, H.P. Kaufmann-Institute, Piusallee 68, D-48147 Münster, Germany |
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Abstract: | The substrate selectivity of several microbial lipases has been examined in the esterification of the conjugated linoleic acid (CLA) isomers cis-9,trans-11-, cis-9,cis-11-, trans-9,trans-11- and trans-10,cis-12-octadecadienoic acid with n-butanol in n-hexane. Lipases from Candida cylindracea and Mucor miehei had a preference for the cis-9,trans-11-octadecadienoic acid, while Chirazyme L-5, a Candida antarctica lipase A, accepted the trans-9,trans-11-fatty acid with a high selectivity. Moreover, lipase from Candida cylindracea and Chirazyme L-5 catalysed the esterification of the cis-9,trans-11-octadecadienoic acid with n-butanol faster than the corresponding reaction of the trans-10,cis-12-fatty acid. |
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Keywords: | cis/trans-isomers conjugated linoleic acid esterification lipase substrate selectivity |
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