Organogenesis in vitro of Nothofagus alpina (P. et E.) Oerst, Fagaceae |
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Authors: | M Jordan J Velozo A M Sabja |
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Institution: | (1) Departamento de Ecología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Casilla, 114-D Santiago, Chile;(2) Bioforest S.A., Valdivia, Chile |
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Abstract: | In vitro shoot and root regeneration of 2-year-old Nothofagus alpina plants was achieved from several types of expiants cultured in vitro on a modified Woody Plant Medium formulation. Multiple shoot formation was obtained from leaf expiants using 0.45–2.27 M thidiazuron and 0.0049–0.098 M indolebutyric acid. Excised axillary buds formed shoots and roots in the presence of 0.0049 M benzyladenine and 2.46 M indolebutyric acid, or in the absence of plant growth regulators. Nodal sections rooted when 2.46 M indolebutyric acid at was supplied in the medium. Subcultured shoots originating from nodal sections showed a high regeneration rate through multiple shoot and root formation.Abbreviations BA
benzyladenine
- GA3
giberellic acid
- IBA
indolebutyric acid
- NAA
-naphthaleneacetic acid
- TDZ
thidiazuron
- WPM
McCown's Woody Plant Medium
- Z
zeatin |
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Keywords: | |
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