Endo-deoxyribonuclease from Streptomyces rimosus |
| |
Authors: | B Vukelić A Ritonja Lj Vitale |
| |
Institution: | (1) Department of Organic Chemistry and Biochemistry, Rudjer Bo kovi Institute, Bijeni ka c. 54, 41001 Zagreb, Croatia;(2) Department of Biochemistry and Molecular Biology, Jozef Stefan Institute, 61001 Ljubljana, Slovenia |
| |
Abstract: | From filtrates of an oxytetracycline-producing culture of Streptomyces rimosus a deoxyribonuclease was purified to homogeneity and determined to be a potent endo-DNase. It is a monomeric, basic protein (Mr 21 000; pI 9.5) stable in a broad pH range but unstable to higher temperature. The enzyme has an absolute requirement for Mg2 + or Mn2 +, and for its full activity requires free SH groups and a low-ionic-strength environment. Its N-terminal primary structure differs from that of other nucleases. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|