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The activity and stability of wheat nitrate reductase in vitro |
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| Authors: | Peter W Jones Máire ní Mhuimhneacháin |
| Institution: | Botany Department, University College, Cork, Ireland |
| Abstract: | The activity and decay characteristics of nitrate reductase from wheat (Triticum aestivum) were studied in crude, partially-purified and highly-purified preparations. The decay of nitrate reductase activity in crude extracts was due to spontaneous dissociation of the enzyme and to the effects of two decay factors, one present in the 0–30% and the other in the 50–70% saturated (NH4)2SO4 fraction of a crude extract. Low rates of factor-mediated NR decay in vitro were associated with high levels of NR activity in vivo. |
| Keywords: | Gramineae wheat nitrate reductase stability NR nitrate reductase MV° reduced methyl viologen DTNB 5 5′-dithiobis (2-nitro-benzoic acid) PMSF phenylmethylsulphonyl fluoride |
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