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Greening-related lipid changes in leaves,protoplasts and a plasmamembrane-enriched fraction of pea
Authors:K. Manoharan  Rajendra Prasad  Sipra Guha-Mukherjee
Affiliation:Plant Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India
Abstract:Light-induced changes in the membrane lipid compositions were studied in pea leaves and in protoplasts and a plasmamembrane-enriched fraction (PMEF)* of pea leaves. PC, PE, PI, PG, PA, MGDG, DGDG and SL were identified as the glycerolipids. The relative levels of various membrane lipids changed due to light-induced greening. There was an increase in the galactolipids of leaves and leaf protoplasts. The galactolipid constituent of the PMEF was very low and showed no change. Among the plasmamembrane phospholipids, PI increased with a concomitant decrease in PC.
Keywords:Leguminosae  pea  light  membrane lipids  protoplasts  plasmamembrane enriched fraction.  DGDG, digalactosyl diglyceride  MGDG, monogalactosyl  diglyceride  PA, phosphatetic acid  PC, phosphatidylcholine  PE, phosphatidylethanolamine  PG, phosphatidylglycerol  PI, phosphatidylinositol  PMEF, plasmamembrane-enriched fraction.
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