The effects of soybean lipoxygenase-1 on chloroplasts from wheat |
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Authors: | Annerose Köckritz Tankred Schewe B Hieke W Hass |
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Institution: | 1. Humboldt-Universität, Sektion Biologie, Bereich Allg. Botanik, DDR-1040 Berlin, G.D.R.;2. Bereich Medizin (Charite), Institut für Physiologische und Biologische Chemie, DDR-1040 Berlin, G.D.R. |
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Abstract: | Chloroplasts were isolated from primary leaves of wheat 12 days after germination and incubated at 25° for 45 min in the dark with soybean lipoxygenase-1. The lipoxygenase action was evident from a weak oxygen uptake of ca 0.18, μmol/hr per mg chloroplast protein. The lipoxygenase treatment caused a marked decrease in the photochemical activity, as measured by the reduction rate of 2,6-dichlorophenolindophenol. However, both the content and composition of the lipids as well as those of total fatty acids remained largely unchanged except for a slight but significant decrease in the total linolenic acid content. It is proposed that soybean lipoxygenase-1 selectively attacks free linolenic acid present in chloroplasts, followed by a chlorophyll-catalysed reaction of hydroperoxylinolenic acid with components of the electron transfer system. |
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Keywords: | Gramineae wheat isolated chloroplasts lipoxygenase lipids fatty acids photosynthetic electron transport |
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