|
|||||
|
|
Functional expression and production of human H-ferritin in Pichia pastoris |
|
| Authors: | Lee Jung-Lim Song Hyung-Seok Kim Hyun-Joong Park Jong-Hwan Chung Dae-Kyun Park Cheon-Seok Jeoung Dooil Kim Hae-Yeong |
| Affiliation: | (1) College of Life Sciences, Kyung Hee University, Suwon, 449-701, Korea;(2) RNA Inc., Suwon, 442-470, Korea;(3) Department of Microbiology, College of Natural Sciences, Kangwon National University, Chuncheon, 200-701, Korea |
| Abstract: | Human heavy chain ferritin (H-ferritin) was cloned from human heart cDNA library and expressed in Pichia pastoris. The H-ferritin transformant was cultivated by fed-batch and the cell mass reached about 52 g cell dry wt l–1 after 150 h. In atomic absorption spectrometry analysis, intracellular content of iron in H-ferritin transformant was measured to 3038 ± 72  g g–1 which was 9.6-fold more than that of control strain. |
| Keywords: | fed batch functional expression human H-ferritin iron Pichia pastoris |
| 本文献已被 PubMed SpringerLink 等数据库收录！ | |