Kinetic studies and characteristics of a modifier of Ca2+-stimulated ATPase

1. The thermostable modifier increases the Mg2+-stimulated ATPase with a parallel decrease of Ca2+-stimulated ATPase. 2. The modifier inhibited Ca2+-stimulated ATPase by diminishing the velocity without any significant effect on the apparent affinity of binding of Ca2+. 3. The inhibition of Ca2+-stimulated ATPase was independent of Ca2+ concentration and could not be reversed by increasing Ca2+ levels. 4. Monovalent cations and calmodulin increased the Mg2+-stimulated ATPase in the presence of the modifier.