Exploitation of the alcohol dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid |
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Authors: | Fossati Elena Polentini Fausto Carrea Giacomo Riva Sergio |
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Affiliation: | Istituto di Chimica del Riconoscimento Molecolare, C.N.R., Via Mario Bianco 9, 20131 Milano, Italy. |
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Abstract: | The performance of a new NADP-regeneration system, based on the use of alcohol dehydrogenase (ADH)-acetone, has been investigated for the regioselective oxidation of cholic acid (1) to 12-ketochenodeoxycholic acid (2). Enzymes stabilities and substrate and/or product inhibitory effects under defined synthetic reaction conditions have been evaluated. The optimized system, based on a 4% w/v solution of 1 in a reaction mixture containing 25% v/v acetone, allowed the preparative scale transformation of 1 into 2 with a 92% conversion. |
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Keywords: | regioselective oxidation 12α‐hydroxysteroid dehydrogenase 12‐ketochenodeoxycholic acid NADP+ regeneration alcohol dehydrogenase bile acids |
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