Establishment of a clonal cell line that differentiates into adipose cells in vitro |
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Authors: | Akiyoshi Hiragun Mayumi Sato Hiromi Mitsui |
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Institution: | (1) Department of Cell Biology, The Tokyo Metropolitan Institute of Medical Science, Honkomagome 3-18-22, Bunkyo-ku, 113 Tokyo, Japan |
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Abstract: | Summary From the fibroblastic cells of murine mammary tumor tissue we isolated a clonal cell line that could be induced to differentiate
into fat-accumulating cells in vitro. Differentiation began after the cultures had reached confluence and was accompanied
by (a) an increase in the incorporation of sodium acetate into the triglyceride (TG) fraction of cellular lipids, (b) a more
than 50-fold increase in cellular TG content per milligram cell-layer protein basis and (c) the cessation of cellular DNA
synthesis. The addition of insulin to the culture medium enhanced lipid accumulation and increased the fraction of cells that
differentiated into adipocytes. When insulin (5 to 10 μg/ml) was added exogenously, 80% or more of the cells were induced
to differentiate into mature adipocytes within 2 weeks. This cell line can be used as a model for mammalian cell differentiation
and also as a convenient material for the study of lipid metabolism in adipocytes.
This work was supported by Grants in Aid from the Ministry of Education, Science and Culture and from the Ministry of Health
and Welfare of Japan. |
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Keywords: | murine fibroblasts preadipose cells in vitro terminal differentiation insulin effect |
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