Properties of genetically overproducedE. coli xylose isomerase |
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Authors: | M. Y. Tucker M. P. Tucker M. E. Himmel K. Grohmann S. M. Lastick |
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Affiliation: | (1) Fermentation Section, Biotechnology Research Branch, Solar Fuels Division, Solar Energy Research Institute, 1617 Cole Blvd., 80401 Golden, CO |
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Abstract: | Summary Xylose isomerase was purified from a transformedE. coli strain (LE392-pRK248/pTXI-1) (Lastick et al., 1986) that overproduces the enzyme by induction of the strong lambda PL promotor. Kinetic data, N-terminal sequence analysis, SDS polyacrylamide gel electrophoresis, size exclusion chromatography and immunodiffusion were used to compare the overproduced enzyme with xylose isomerase purified from xylose induced, non-transformedE. coli LE392 cells; no differences between these purified enzyme preparations were found. |
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