Characterization of a CV-1 cell cycle. III. Biophysical parameters. |
| |
Authors: | R M Zucker R M D'Alisa E L Gershey |
| |
Affiliation: | Departments of Microbiology and Chemistry, University of Manitoba, Winnipeg, Manitoba, Canada, R3T 2N2 |
| |
Abstract: | Membrane preparations from three independently selected concanavalin A-resistant cell lines incorporated sinificantly less GDP-[14C]mannose into lipid, oligosaccharide-lipid and protein fractions than preparations obtained from parental wild populations. The results from experiments with membranes from a revertant concanavalin A-resistant line more closely resembled the wild-type populations. The amount of mannose label incorporated into glycoprotein in the variant cells was higher than expected if it is assumed that the pathway GDP-mannose → mannolipid → oligosaccharide-lipid → mannoprotein is functioning in these cells. Evidence is presented to suggest that conversion of mannose label to fucose occurs in wild-type and variant cell lines and that this pathway may be of greater importance in the variant cells; this result could explain at least in part, the higher than expected levels of 14C-label in glycoprotein in the variant cell lines. The changes in the glycosyl transferase activities in these lectin-resistant cell lines are probably involved in determining the concanavalin A-resistant property and the accompanying complex phenotype exhibited by these variant cell lines. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|