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Transcutaneous immunization with hydrophilic recombinant gp100 protein induces antigen-specific cellular immune response |
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| Authors: | Eisenberg Galit Machlenkin Arthur Frankenburg Shoshana Mansura Adva Pitcovski Jacob Yefenof Eitan Peretz Tamar Lotem Michal |
| Affiliation: | a Sharett Institute of Oncology, Hadassah Medical Organization, P.O. Box 12000, Jerusalem 91120, Israel b Dermatology Department, Hadassah Medical Organization, P.O. Box 12000, Jerusalem 91120, Israel c Migal, Kiryat Shmona, Tel Hai Academic College, Upper Galilee, Israel d Lautenberg Center for General and Tumor Immunology, Hebrew University of Jerusalem, Jerusalem 91120, Israel |
| Abstract: | The objective of this study was to evaluate the potential of transcutaneous immunization with tumor antigen to induce cell-mediated immunity. For this purpose, hydrophilic recombinant gp100 protein (HR-gp100) was topically applied on human intact skin in vitro, and used as a vaccine in a mouse model. We demonstrate that HR-gp100 permeates into human skin, and is processed and presented by human dendritic cells. In a mouse model, an HR-gp100-based vaccine triggered antigen-specific T cell responses, as shown by proliferation assays, ELISA and intracellular staining for IFN-γ. Transcutaneous antigen delivery may provide a safe, simple and effective method to elicit cell-mediated immunity. |
| Keywords: | Transcutaneous immunization gp100 Cancer vaccine |
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